Mushroom Growing
Temperature Chart

Temperature
(Incubation)
Temperature
(Fruiting)
Humidity
(Incubation)
Humidity
(Fruiting)
CO₂ Levels
(Fruiting)
Light
Requirements
Substrate Key Notes
Button Mushrooms
(Agaricus bisporus)
20–24°C
(68–75°F)
13–16°C
(55–60°F)
70% RH 85–95% RH <1,000 ppm None required Composted
manure/straw
Requires strict temperature control; casing layer essential for fruiting
Oyster Mushrooms
(Pleurotus ostreatus)
20–24°C
(68–75°F)
15–21°C
(59–70°F)
70% RH 85–95% RH <800 ppm 12–16 hr/day
indirect
Straw/hardwood
sawdust
Tolerates higher CO₂ (up to 1,100 ppm for some strains). Fast colonization (10–14 days)
Shiitake
(Lentinula edodes)
20–24°C
(68–75°F)
13–18°C
(55–64°F)
70% RH 85–95% RH <1,100 ppm 10–12 hr/day Hardwood
logs/sawdust
Requires thermal shock (10–15°C) to initiate fruiting. Slow colonization (3 weeks)
Enoki
(Flammulina velutipes)
20–24°C
(68–75°F)
13–15°C
(55–59°F)
70% RH 90–95% RH <1,000 ppm None required Hardwood
sawdust
Cold shock (4–7°C for 24–48 hr) triggers fruiting. Grows in high-density clusters
Lion's Mane
(Hericium erinaceus)
20–24°C
(68–75°F)
18–24°C
(64–75°F)
70% RH 85–90% RH <1,100 ppm 12–14 hr/day Hardwood
substrates
Sensitive to low humidity; produces icicle-like spines
Pink Oyster
(Pleurotus djamor)
24–28°C
(75–82°F)
20–30°C
(68–86°F)
70% RH 85–90% RH <1,000 ppm 12–14 hr/day Straw/coffee
grounds
Tropical species; tolerates higher temperatures but prone to contamination
Morel
(Morchella spp.)
15–20°C
(59–68°F)
10–15°C
(50–59°F)
85% RH 90–95% RH <500 ppm Natural daylight
cycles
Nutrient-rich
soil
Challenging to cultivate; often requires symbiotic tree roots

Wanna know more?

You can check our guides on growing your own mushrooms. There you'll find specific tips, techniques and everything you need to know in order to set up your own mushroom farm, either as a hobby or for commercial purposes: